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Image Search Results
Journal:
Article Title: The SANT2 Domain of the Murine Tumor Cell DnaJ-like Protein 1 Human Homologue Interacts with ? 1 -Antichymotrypsin and Kinetically Interferes with Its Serpin Inhibitory Activity
doi: 10.1074/jbc.M310903200
Figure Lengend Snippet: A, schematic representation of HTJ1 constructs used in this study. The position of amino acids in HTJ1, the size in kDa, and the interaction with ACT are indicated. B, immunoblot of total yeast protein extracts. Protein extracts from the AH109 yeast strain transformed with (lane 1), pGBKT7/pGBKT7/HTJ1242–554 HTJ1242–493 (lane 2), pGBKT7/HTJ1493–554 (lane 3), pGBKT7/HTJ1493–554:W497A (lane 4), pGBKT7/HTJ1493–554:W520A (lane 5), pGBKT7/HTJ1493–554:W497A, W520A (lane 6), pGBKT7/HTJ1242–411 (lane 7), pGBKT7/HTJ145–148 (lane 8), and pGBKT7 vector (lane 9) were separated on a 10% SDS-PAGE gel and detected with an anti-c-Myc monoclonal antibody.
Article Snippet: All components of the two-hybrid system, including cloning vectors pGBKT7 and pACT2, human liver cDNA library, S. cerevisiae strain AH109,
Techniques: Construct, Western Blot, Transformation Assay, Plasmid Preparation, SDS Page